National Botanical Research Institute, Lucknow & University of Lucknow, Lucknow
DBT Ramalingaswami fellow (Oct. 2013- March 2017),Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, UP
Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, UP
Having keen interest towards understanding how the process of gene expression is controlled in higher organisms i.e. mammals/plants at molecular level, I joined the laboratory of Dr. Rakesh Tuli, at the National Botanical Research Institute, Lucknow, India to pursue PhD in 2001. My PhD training expanded my horizon in understanding the basic concepts of regulation of gene expression at transcriptional and chromatin levels. I was successful in applying some of the basic concepts well towards developing synthetic promoters and expression vectors that can be used to tightly control transgenes expression in regulated and tissue specific manner in plants. While I was on the verge of completing my doctoral degree, I got more inclined to take up research in the area biomedical sciences involving stem cells. I felt that stem cells are the centre stage for future therapeutic breakthroughs as they can differentiate into all cell types and could potentially be used in developing therapies against cancer, leukemia, thalassemia, sickle cell anemia and degenerative diseases. I joined Dr. Marjorie Brand’s laboratory at the Sprott Center for Stem Cell Research (Ottawa Health Research Institute) as Postdoctoral fellow in Ottawa in 2007. A stay of over six years in her laboratory provided me an excellent background in the developmental aspects of hematopoiesis, hematological disorders epigenetics and stem cell biology. On technical front, I successfully applied ICAT technique of quantitative proteomics to study gene expression regulation in biological systems and developed skill in other highly demanding genomic techniques like expression microarray, RNAi, modern chromatin related techniques like ChIP, native ChIP, ChIP sequencing and culture of leukemic cell lines and primary human hematopoietic stem cells. After the completion of over six years of postdoctoral training, I returned back in 2013 and joined Stem Cell Research Facility of Department of Hematology at Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS), Lucknow as DBT-Ramalingaswami Fellow. The focus of my research at SGPGIMS is to study the role of epigenetic regulators (involved in histone methylases/demethylases) in normal hematopoiesis and how the mutations in these epigenetic regulators alter their functional properties and contribute in inducing leukemogenesis.