Publications and Presentations

48. Illuminating GPCR Signaling by Cryo-EM. Safdari HA, Pandey S, Shukla AK, Dutta S. Trends in Cell Biol. 2018 Jun 23.

47. Molecular mechanism of modulating arrestin conformation by GPCR phosphorylation. Sente A, Peer R, Srivastava A, Baidya M, Lesk AM, Balaji S, Shukla AK, Babu MM, Flock T. Nature Structure & Molecular Biology. 2018 Jun;25(6):538-545.

46. Emerging Paradigm of Intracellular Targeting of G Protein-Coupled Receptors. Chaturvedi M, Schilling J, Beautrait A, Bouvier M, Benovic JL, Shukla AK. Trends in Biochemical Sciences. 2018 Jul;43(7):533-546.

45. GPCR Signaling: The Interplay of Gαi and β-arrestin. Dwivedi H, Baidya M, Shukla AK. Current Biology. 2018 Apr 2;28(7):R324-R327.  

44. A synthetic intrabody-based selective and generic inhibitor of GPCR endocytosis. Ghosh E, Srivastava A, Baidya M, Kumari P, Dwivedi H, Nidhi K, Ranjan R, Dogra S, Koide A, Yadav PN, Sidhu SS, Koide S, Shukla AK. Nature Nanotechnology. 2017 , Oct 2. doi: 10.1038/nnano.2017.188.

43. Novel insights into GPCR-β-arrestin interaction and signaling. Ranjan R, Dwivedi H, Baidya M, Kumar M, Shukla AK. Trends in Cell Biology. 2017, Nov;27(11):851-862.

42. Frozen in action: cryo-EM structure of a GPCR–G-protein complex. Baidya M, Dwivedi H, Shukla AK. Nature Structural & Molecular Biology. 2017, Jun 6;24(6):500-502.

41. Core engagement with β-arrestin is dispensable for agonist-induced vasopressin receptor endocytosis and ERK activation. Kumari P, Srivastava A, Ghosh E, Ranjan R, Dogra S, Yadav PN, Shukla AK. Mol Biol Cell. 2017 Apr 15;28(8):1003-1010

40. Distinct conformations of GPCR-β-arrestin complexes mediate desensitization, signaling, and endocytosis. Cahill TJ 3rd, Thomsen AR, Tarrasch JT, Plouffe B, Nguyen AH, Yang F, Huang LY, Kahsai AW, Bassoni DL, Gavino BJ, Lamerdin JE, Triest S, Shukla AK, Berger B, Little J 4th, Antar A, Blanc A, Qu CX, Chen X, Kawakami K, Inoue A, Aoki J, Steyaert J, Sun JP, Bouvier M, Skiniotis G, Lefkowitz RJ. Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):2562-2567.

39. Biased Opioid Receptor Ligands: Gain without Pain. Ranjan R, Pandey S, Shukla AK. Trends Endocrinol Metab. 2017 Apr;28(4):247-249.

38. Functional competence of a partially engaged GPCR-β-arrestin complex. Kumari P, Srivastava A, Banerjee R, Ghosh E, Gupta P, Ranjan R, Chen X, Gupta B, Gupta C, Jaiman D, Shukla AK. Nat Commun. 2016 Nov 9;7:13416.

37. GPCR-G Protein-β-Arrestin Super-Complex Mediates Sustained G Protein Signaling. Thomsen AR, Plouffe B, Cahill TJ 3rd, Shukla AK, Tarrasch JT, Dosey AM, Kahsai AW, Strachan RT, Pani B, Mahoney JP, Huang L, Breton B, Heydenreich FM, Sunahara RK, Skiniotis G, Bouvier M, Lefkowitz RJ. Cell. 2016 Aug 11;166(4):907-19.

36. Ranjan R, Gupta P, Shukla AK*.  GPCR Signaling: β-arrestins Kiss and Remember. Curr Biol. 2016 Apr 4;26(7):R285-8.

35. Kumari P, Ghosh E, Shukla AK*. Emerging Approaches to GPCR Ligand Screening for Drug Discovery. Trends Mol Med. 2015 Nov;21(11):687-701.

34. Srivastava A, Gupta B, Gupta C, Shukla AK*. Emerging Functional Divergence of β-Arrestin Isoforms in GPCR Function. Trends Endocrinol Metab. 2015 Nov;26(11):628-42.

33. Shukla AK*, Kumari P, Ghosh E and Kumari N. From Recombinant Expression to Crystals: A Step-by-Step Guide to GPCR Crystallography. Methods in Enzymology.  2015;556:549-61.   

32. Shukla AK*, Gupta C, Srivastava A and Jaiman D. Antibody Fragments for Stabilization and Crystallization of G Protein-Coupled Receptors and Their Signaling complexes. Methods in Enzymology. 2015;557:247-58

31. Ghosh E, Kumari N, Shukla AK*. GPCR-Ligand Interactions. Cell, 2014 Dec 18;159(7):1712-1712.e1.

30. Ghosh E, Kumari P, Jaiman D, Shukla AK*. Methodological advances: the unsung heroes of the GPCR structural revolution. Nat Rev Mol Cell Biol. 2015 Feb;16(2):69-81.

29. Shukla AK*, Singh G, Ghosh E. Emerging Structural Insights Into Biased GPCR Signaling. Trends in Biochem. Sci. 2014 Vol. 39, Issue 12, p594–602.

28. Shukla AK#, Westfield GH#, Xiao K#, Reis RI, Huang LY, Tripathi-Shukla P, Qian J, Li S, Blanc A, Oleskie AN, Dosey AM, Su M, Liang CR, Gu LL, Shan JM, Chen X, Hanna R, Choi M, Yao XJ, Klink BU, Kahsai AW, Sidhu SS, Koide S, Penczek PA, Kossiakoff AA, Woods VL Jr, Kobilka BK, Skiniotis G, Lefkowitz RJ. Visualization of arrestin recruitment by a G-protein-coupled receptor. Nature, 2014 Aug 14;512(7513):218-22.

27. Aristotelous T#, Ahn S#, Shukla AK#, Gawron S, Sassano MF, Kahsai AW, Wingler LM, Zhu X, Tripathi-Shukla P, Huang XP, Riley J, Besnard J, Read KD, Roth BL, Gilbert IH, Hopkins AL, Lefkowitz RJ, Navratilova I. Discovery of β2 Adrenergic Receptor Ligands Using Biosensor Fragment Screening of Tagged Wild-Type Receptor. ACS Med Chem Lett. 2013 Oct 10;4(10):1005-1010.

26. Shukla AK#, Manglik A#, Kruse AC#, Xiao K, Reis RI, Tseng W, Staus DP, Hilger D, Uysal S, Huang L, Paduch M, Tripathi-Shukla P, Koide A, Koide S, Weis WI, Kossiakoff AA, Kobilka BK, Lefkowitz RJ. Structure of active Beta-Arrestin1 bound to a G protein-coupled receptor phosphopeptide. Nature, 2013 May 2;497(7447):137-41.  

25. Shukla AK*. Biasing GPCR signaling from inside. Sci Signal. 2014 Jan 28;7(310):pe3.

24. Reiter E, Ahn S, Shukla AK, Lefkowitz RJ. Molecular mechanism of β-arrestin-biased agonism at seven-transmembrane receptors. Annu Rev Pharmacol Toxicol. 2012 Feb 10;52:179-97.

23. Shukla AK*, Xiao K, Lefkowitz RJ. Emerging paradigms of beta arrestin dependent seven transmembrane rteceptor signaling. Trends in Biochem Sci. 2011 Sep;36(9):457-69. Featured Article.

22. Nobles K, Xiao K, Ahn S, Shukla AK, Lam C, Rajagopal S, Bressler E, Hara M, Shenoy S, Gygi S, Lefkowitz RJ. Distinct phosphorylation sites on the β(2)-adrenergic receptor establish a barcode that encodes differential functions of β-arrestin. Science Signaling 2011 Aug 9;4(185):ra51.

21. Kahsai AW, Xiao K, Rajagopal S, Ahn S, Shukla AK, SunJ, Oas T, Lefkowitz RJ. Multiple Ligand-Specific Conformations of the β2-Adrenergic Receptor. Nature Chemical Biology. 2011 Aug 21;7(10):692-700. Recommended by Faculty of 1000.

20. Xiao K, Sun J, Kim J, Rajagopal S, Zhai B, Villén J, Haas W, Kovacs JJ, Shukla AK, Hara MR, Hernandez M, Lachmann A, Zhao S, Lin Y, Cheng Y, Mizuno K, Ma'ayan A, Gygi SP, Lefkowitz RJ. Global phosphorylation analysis of beta-arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR). Proc Natl Acad Sci U S A. (2010) Aug 24;107(34):15299-304.

19. Shukla AK*#, Kim J#, Ahn S, Xiao K, Shenoy SK, Liedtke W, Lefkowitz RJ.Arresting a transient receptor potential (TRP) channel: beta-arrestin 1 mediates ubiquitination and functional downregulation of TRPV4. Journal of Biological Chemistry. (2010) Sep 24;285(39):30115-25.

This article was selected as “Paper of the Week” by Journal of Biological Chemistry. It was also highlighted under “Editor’s Choice” section by Science Signaling.

18. Mangmool S, Shukla AK, Rockman HA. beta-Arrestin-dependent activation of Ca(2+)/calmodulin kinase II after beta(1)-adrenergic receptor stimulation. Journal of Cell Biology. (2010) May 3;189(3):573-87.

17. Shenoy SK, Modi AS, Shukla AK, Xiao K, Berthouze M, Ahn S, Wilkinson KD, Miller WE, Lefkowitz RJ. Beta-arrestin-dependent signaling and trafficking of 7-transmembrane receptors is reciprocally regulated by the deubiquitinase USP33 and the E3 ligase Mdm2. Proc Natl Acad Sci U S A. (2009) Apr 21;106(16):6650-5.

16. Walters RW, Shukla AK, Kovacs JJ, Violin JD, DeWire SM, Lam CM, Chen JR, Muehlbauer MJ, Whalen EJ, Lefkowitz RJ. beta-Arrestin1 mediates nicotinic acid-induced flushing, but not its antilipolytic effect, in mice. Journal of Clinical Investigation. (2009) May;119(5):1312-21.

15. Shukla AK, Violin JD, Whalen EJ, Gesty-Palmer D, Shenoy SK, Lefkowitz RJ. Distinct conformational changes in beta-arrestin report biased agonism at seven-transmembrane receptors. Proc Natl Acad Sci U S A. (2008) Jul 22;105(29):9988-93.

14. Lefkowitz RJ, Sun JP, Shukla AK. A crystal clear view of the beta2-adrenergic receptor. Nature Biotechnology, (2008) Feb;26(2):189-91.

13. Shukla AK, Sun JP, Lefkowitz RJ. Crystallizing thinking about the beta2-adrenergic receptor. Mol Pharmacol. (2008) May;73(5):1333-8.

12. Lopez JJ, Shukla AK, Reinhart C, Schwalbe H, Michel H, Glaubitz C. The structure of the neuropeptide bradykinin bound to the human G-protein coupled receptor bradykinin B2 as determined by solid-state NMR spectroscopy. Angew Chem Int Ed Engl. (2008) 47(9):1668-71. This article was featured on the cover page of the journal.

11. Roy A, Shukla AK, Haase W, Michel H. Employing Rhodobacter sphaeroides to functionally express and purify human G protein-coupled receptors. Biol Chem. (2008) Jan;389(1):69-78.

10. Shukla AK*, Haase W, Reinhart C, Michel H*. Heterologous expression and characterization of the recombinant bradykinin B2 receptor using the methylotrophic yeast Pichia pastoris. Protein Expr Purif. (2007) Sep;55(1):1-8.

9. Shenoy SK, Barak LS, Xiao K, Ahn S, Berthouze M, Shukla AK, Luttrell LM, Lefkowitz RJ. Ubiquitination of beta-arrestin links seven-transmembrane receptor endocytosis and ERK activation. J Biol Chem. (2007) Oct 5;282(40):29549-62.

8. Xiao K, McClatchy DB, Shukla AK, Zhao Y, Chen M, Shenoy SK, Yates JR 3rd, Lefkowitz RJ. Functional specialization of beta-arrestin interactions revealed by proteomic analysis. Proc Natl Acad Sci U S A. (2007) Jul 17;104(29):12011-6. Recommended by Faculty of 1000.

7. Shukla AK*, Haase W, Reinhart C, Michel H*. Heterologous expression and comparative characterization of the human neuromedin U subtype II receptor using the methylotrophic yeast Pichia pastoris and mammalian cells. Int J Biochem Cell Biol. (2007) 39(5):931-42.

6. Shukla AK*, Reinhart C, Michel H*. Comparative analysis of the human angiotensin II type 1areceptor heterologously produced in insect cells and mammalian cells. Biochem Biophys Res Commun. (2006) Oct 13;349(1):6-14.  

5. Shukla AK*, Reinhart C, Michel H*. Dimethylsulphoxide as a tool to increase functional expression of heterologously produced GPCRs in mammalian cells. FEBS Lett. (2006) Jul 24;580(17):4261-5.

4. Shukla AK*, Haase W, Reinhart C, Michel H*. Biochemical and pharmacological characterization of the human bradykinin subtype 2 receptor produced in mammalian cells using the Semliki Forest virus system. Biol Chem. (2006) May;387(5):569-76.

3. Shukla AK*, Haase W, Reinhart C, Michel H*. Functional overexpression and characterization of human bradykinin subtype 2 receptor in insect cells using the baculovirus system. J Cell Biochem. (2006) Oct 15;99(3):868-77.  

2. Shukla AK*, Roy KB*. Rec A-independent homologous recombination induced by a putative foldback tetraplex DNA. Biol Chem. (2006) Mar;387(3):251-6.

1. Shukla AK, Roy KB. A palindromic repeat sequence adopts a stable fold back structure under supercoiling. J Biochem. (2006) Jan;139(1):35-9.