Research Summary

Understanding mechanisms of vesicle release at the endocytic recycling compartment

Internalization of membrane receptors from the cell surface is vital to cellular physiology since it regulates nutrient uptake and display of adhesion molecules, ion-channels and antigen-presenting receptors. Internalized receptors are sent to the lysosome for degradation or recycled back to the cell surface for additional rounds of endocytosis. The latter process is known as endocytic recycling and is managed by the endocytic-recycling compartment (ERC). The mechanism by which vesicles are released from the ERC remains unclear. We propose to address this question by taking a reconstitution approach to understand how proteins remodel and vesiculate membranes and advance into animal cell culture systems or model organisms to appreciate the significance of this pathway to cellular and organismal physiology.