Research Summary

Understanding Salmonella-mediated alterations in host SUMOylation:implications in infection and inflammation

Salmonella Typhimurium is one of the most frequent causes of acute gastroenteritis in humans. Moreover, Salmonellosis simulates ulcerative colitis clinically, histopathologically, and radiologically and therefore is very good model organism to study inflammation of gut. During infection Salmonella manipulates host cells in various ways to allow its own replication, propagation, escape from host defense and dissemination using several effector proteins. In my lab we focus to understand these complex mechanisms at their molecular details. We have obtained preliminary data that suggest Salmonella infection alters host SUMOylation machinery, a post-translational modification pathway that is otherwise unknown in the field. We hypothesize that modulation of host SUMOylation could be a crucial mechanism leading to the disease. We intend to elucidate and characterize the role of SUMOylation in inflammation using Salmonella as a model. Our strategy involves screening and characterization entire SUMO-proteiome that display alteration during infection. Ultimately the significance of the mechanism will be validated and characterized in models and conditions of auto-immune disorders of the gut. Understanding of this novel biological phenomenon will allow us to dissect out aspects of inflammatory-pathway that is yet to be documented.

Figure Legend: H&E stained Intestinal section of mutant Salmonella infected mice