Research SummaryInvestigating cellular processes and pathways controlled by phosphatases
Phosphatases are a group of ubiquitously expressing enzymes, which are responsible for the removal of a phosphate group of various proteins in a cell. Phosphatases play a crucial role in nearly every cellular process, including metabolism, gene transcription and translation, cell-cycle progression, protein stability, signal transduction, and apoptosis. While several kinases (their counterpart enzymes) have been found to be intricately involved in human malignancies, studies on the role of phosphatases are very limited.
With the support of Wellcome Trust/DBT India Alliance Intermediate fellowship, we established a detailed interaction network of human phosphatases derived by a systematic proteomic analysis of 143 phosphatases using tandem affinity purification coupled with mass spectrometry. Based on our phosphatase interactome, we propose to identify novel cellular functions for different phosphatases and characterize their importance in various cellular pathways as well as assign roles in human malignancies with the following aims:
Aim 1: Identification of new cellular functions for phosphatases.
Aim 2: Characterizing the functional role of phosphatases in different cellular pathways
Aim 3: Investigate the phosphatase interactions linked with human malignancies.
In the current project, we expect to identify novel cellular functions and pathways regulated by phosphatases along with their role in human cancers.