Research Summary

RNAi screen for novel genes regulating self-renewal and pluripotency of embryonic stem (ES) cells

I am using RNAi based screens to identify novel factors regulating pluripotency of ES cells. The novel genes identified in the RNAi screens will be studied intensively to under stand their role in regulation of pluripotency. I am using the loss of function (Gene Knockout mice), gain of function and biochemical approaches to understand the molecular mechanisms by which the novel factors regulate pluripotency. This understanding may be useful to design, develop or optimise methods to efficiently and deliberately differentiate ES cells to specific cell types or lineages.