Research SummaryNanomaterial Hazard Identification: High throughput toxicity assessment using Zebra fish model
Rapid growth of nanotechnology in the past decade has produced hundreds of new engineered nanomaterials (ENM), which are steadily leaving the laboratory and entering the market, bringing with them the real possibility of exposure to global populations and ecosystems in general. The large variety of ENM, coupled with their novel physicochemical and biological properties, have posed an urgent need to develop timely, high throughput, cost efficient, and predictive toxicity screening/evaluation approaches of ENM. The animal studies using rodents are expensive and time consuming therefore zebra fish embryo model due to its unique features may provide a better alternate for in-vivo studies. This model has the potential to fit in between the traditional cell culture and mammalian models, providing validation of in vitro-toxicity and prioritization of in-vivo animal experiments.
In this project, we intend to use zebra fish embryo model for in-vivo high throughput toxicity screening of assorted ENMs from different classes and validate its utility compared to various in-vitro systems. At present most of the toxicity research conducted on nano-based products pertains to short duration only, with miniscule prediction of long-term toxicity. Hence, the proposed study will attempt to answer some of the questions related to chronic toxicity.
Figure Legend: Nanomaterial hazard identification using zebra fish