Research Summary

Training Programme: Isolation and in vitro and in vivo characterization of cancer stem cells from human osteosarcoma tissue and assessment of chemo sensitivity

Osteosarcoma (OS) is the most common primary tumor of the bone in children characterized by heterogeneity and variability in the chemo sensitivity resulting in the high rate of recurrence. Similar to other malignancies, the concept of Cancer stem cells (CSCs) is being debated and discussed for OS, mainly in OS cell lines. CSCs have the property of self-renewal, anchorage independence and plasticity and may be responsible for chemoresistance and metastasis which makes them a potential therapeutic target.

This study is being conducted to explore the concept of CSCs in primary OS pertaining to the clinical perspective of the management strategy at the time of diagnosis.

Tumour cells (TC) have been isolated from the primary OS tissue procured from biopsy samples and excised tumours in three children with a mean age of 8 yrs. CSCs have been isolated using tumour spheroids and aldehyde dehydrogenase (ALDH) method in one sample. In Vitro characterization of TCs and CSCs using stemness markers is being carried out. Xenograft in SCID mice are underway for one each sample of TCs and CSCs. Chemosensitivity protocols are being standardized using cell line before applying to primary cells. 

Figure Legend: Tumour Spheres formed by Primary osteosarcoma cells. (Day 4, 10x, Phase contrast)