Research Summary

Title of the Training Programme: Role of Extracellular Matrix in Modulating Therapeutic Response of Mesenchymal Stem Cells in Fracture Healing

 

                                                                                  

                       Principal Supervisor:                                                                     Co- Supervisor:

                       Dr. Aditya Chaubey                                                                        Dr. Paul C Salins

     Mazumdar Shaw Center for Translational Research,     Mazumdar Shaw Medical Center, Bangalore, India

     Bangalore, India                          

About 10%-20% of all fractures result in non-union and exhibit markedly impaired healing. Recent evidence supports the role of mesenchymal stem cells (MSC) in bone healing. However, current attempts to expand MSCs ex vivo have largely been unsuccessful because of the loss of their stem cell phenotype during culture. The loss of MSC properties when cultured in vitro on 2D surface strongly suggests that a critical feature of the marrow environment responsible for the maintenance of MSC ‘stemness’ is missing in standard culture systems. We postulate that it is the lack of an appropriate extracellular matrix (ECM) that is responsible for the loss of ‘stemness’ of MSCs when cultured on a standard tissue culture plastic surface. Further, we hypothesize that introducing MSCs cultured on a scaffold derived from key ECM molecules will lead to enhanced fracture healing        

                                                              

                                                                                                                                                    

 

Figure Legend: Flow chart illustrating the study design aimed at understanding the efficacy of scaffold derived from extracellular matrix components combined with mesenchymal stem cells in fracture healing.